The resolution of five linker histone subtypes from chicken erythrocytes

FEBS Letters

Harborne, Nerina¹  Allan, James¹ 

[1] Department of Biophysics, Kings College, Strand, London WC2R2LS, England

关键词: (Chicken erythrocyte);    Linker histone;    Histone H1 subtype;    Histone H5;    CMC-25 Sephadex;   

DOI  :  10.1016/0014-5793(86)80098-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A method is described for the isolation of 4 highly purified H1 linker histone subfractions, and histone H5, from chicken erythrocytes. Fractionation is achieved under non-denaturing conditions by elution from CMC-25 Sephadex with an NaCl gradient. When applied to calf thymus H1, the order of subtype elution differs from that acheived on Amberlite IRC-50 [(1966) J. Biol. Chem. 241, 5790-5797; (1976) Biochemistry 15, 4233-4242]. The two column types employed in series could provide a means for improving subtype purity.

【 授权许可】

Unknown