期刊论文详细信息
FEBS Letters | |
The resolution of five linker histone subtypes from chicken erythrocytes | |
Harborne, Nerina1  Allan, James1  | |
[1] Department of Biophysics, Kings College, Strand, London WC2R2LS, England | |
关键词: (Chicken erythrocyte); Linker histone; Histone H1 subtype; Histone H5; CMC-25 Sephadex; | |
DOI : 10.1016/0014-5793(86)80098-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A method is described for the isolation of 4 highly purified H1 linker histone subfractions, and histone H5, from chicken erythrocytes. Fractionation is achieved under non-denaturing conditions by elution from CMC-25 Sephadex with an NaCl gradient. When applied to calf thymus H1, the order of subtype elution differs from that acheived on Amberlite IRC-50 [(1966) J. Biol. Chem. 241, 5790-5797; (1976) Biochemistry 15, 4233-4242]. The two column types employed in series could provide a means for improving subtype purity.
【 授权许可】
Unknown
【 预 览 】
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