FEBS Letters | |
Antibody against globular domain of H1° histone | |
Morton Bradbury, E.1  Yasuda, Hideyo1  Logan, Kathleen Ann1  | |
[1] Department of Biological Chemistry, School of Medicine, University of California, Davis, CA 95616, USA | |
关键词: Histone H1°; Histone H5; Erythrocyte differentiation; Immunoblotting; GH1; GH1°; GH5; globular H1; H1° and H5; | |
DOI : 10.1016/0014-5793(84)80092-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Antibodies against the globular domain of histones H1° and H5 were developed in rabbit. The antibody against the globular domain of H5 cross-reacted with H1° but not with H1; the antibody against the globular domain of H1° did not cross-react with H5, H1 or with HMG proteins. The globular domain of H1° therefore appears to have an immunological determinant(s) which does not exist in H1 and H5. By use of these antibodies, we show that nucleated erthrocytes of bullfrog contain an H1°-like protein (not an H5-like protein). This observation coincided with the report of Shimada, T. [J. Biol. Chem. 256 (1981) 10577–10582]. These antibodies have application in detecting H1°-like proteins in eukaryotic cells.
【 授权许可】
Unknown
【 预 览 】
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