Antibody against globular domain of H1° histone

FEBS Letters

Morton Bradbury, E.¹  Yasuda, Hideyo¹  Logan, Kathleen Ann¹ 

[1] Department of Biological Chemistry, School of Medicine, University of California, Davis, CA 95616, USA

关键词: Histone H1°;    Histone H5;    Erythrocyte differentiation;    Immunoblotting;    GH1;    GH1°;    GH5;    globular H1;    H1° and H5;   

DOI  :  10.1016/0014-5793(84)80092-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies against the globular domain of histones H1° and H5 were developed in rabbit. The antibody against the globular domain of H5 cross-reacted with H1° but not with H1; the antibody against the globular domain of H1° did not cross-react with H5, H1 or with HMG proteins. The globular domain of H1° therefore appears to have an immunological determinant(s) which does not exist in H1 and H5. By use of these antibodies, we show that nucleated erthrocytes of bullfrog contain an H1°-like protein (not an H5-like protein). This observation coincided with the report of Shimada, T. [J. Biol. Chem. 256 (1981) 10577–10582]. These antibodies have application in detecting H1°-like proteins in eukaryotic cells.

【 授权许可】

Unknown