FEBS Letters | |
Fusion of Sendai virions with phosphatidylcholine‐cholesterol liposomes reflects the viral activity required for fusion with biological membranes | |
Citovsky, Vitaly1  Loyter, Abraham1  Blumenthal, Robert2  | |
[1] Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel;Section on Membrane Structure and Function, Laboratory of Theoretical Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20205, USA | |
关键词: Liposome; Membrane fusion; Sendai virus; Reconstitution; | |
DOI : 10.1016/0014-5793(85)80137-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Sendai virus envelopes were reconstituted after solubilization of intact virions with either Triton X-100 or octylglucoside. Envelopes obtained from Triton X-100, but not from octylglucoside solubilized virions, were hemolytic and promoted cell-cell fusion. Fluorescence dequenching studies [using N-4-nitrobenzo-2-oxa-1,3-diazole phosphatidylethanolamine-bearing viral envelopes] revealed that both preparations fused with negatively charged phospholipids. Fusion with phosphatidylcholine (PC)/cholesterol (chol) liposomes was promoted only by the hemolytic viral envelopes. Fluorescence dequenching studies, using intact virions bearing octadecylrhodamine B chloride, revealed that intact virions fused with PC/chol as well as with negatively charged phospholipids. Only fusion with PC/chol liposomes was inhibited by phenylmethylsulfonyl fluoride and dithiothreitol, reagents which are known to block the viral ability to fuse with biological membranes.
【 授权许可】
Unknown
【 预 览 】
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