Fusion of Sendai virions with phosphatidylcholine‐cholesterol liposomes reflects the viral activity required for fusion with biological membranes

FEBS Letters

Citovsky, Vitaly¹  Loyter, Abraham¹  Blumenthal, Robert² 

[1] Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel;Section on Membrane Structure and Function, Laboratory of Theoretical Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20205, USA

关键词: Liposome;    Membrane fusion;    Sendai virus;    Reconstitution;   

DOI  :  10.1016/0014-5793(85)80137-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sendai virus envelopes were reconstituted after solubilization of intact virions with either Triton X-100 or octylglucoside. Envelopes obtained from Triton X-100, but not from octylglucoside solubilized virions, were hemolytic and promoted cell-cell fusion. Fluorescence dequenching studies [using N-4-nitrobenzo-2-oxa-1,3-diazole phosphatidylethanolamine-bearing viral envelopes] revealed that both preparations fused with negatively charged phospholipids. Fusion with phosphatidylcholine (PC)/cholesterol (chol) liposomes was promoted only by the hemolytic viral envelopes. Fluorescence dequenching studies, using intact virions bearing octadecylrhodamine B chloride, revealed that intact virions fused with PC/chol as well as with negatively charged phospholipids. Only fusion with PC/chol liposomes was inhibited by phenylmethylsulfonyl fluoride and dithiothreitol, reagents which are known to block the viral ability to fuse with biological membranes.

【 授权许可】

Unknown