Effects of protease inhibitors on nuclear binding of glucocorticoid hormones in C3H10T ½ cells

FEBS Letters

Umans, Robert S.¹  Kennedy, Ann R.¹  Radner, Babette¹ 

[1] Department of Cancer Biology, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA

关键词: Glucocorticoid hormone;    Protease inhibitor;    Malignant transformation;    Nuclear binding;    Hormone-receptor complex;   

DOI  :  10.1016/0014-5793(85)80072-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have measured incorporation of the glucocorticoid hormone cortisone into nuclear hormone-receptor complexes in the C3H10T ½ cell line. As we had found cortisone to be capable of malignantly transforming these cells in vitro, and certain protease inhibitors have been shown to suppress transformation in this cell line, we investigated the effects of these protease inhibitors (antipain, chymostatin and the Bowman-Birk inhibitor) on the formation of nuclear cortisone-receptor complexes. All 3 inhibitors were found to suppress wholly or partially formation of nuclear cortisone-receptor complexes, suggesting that such complexes may be involved in the process of glucocorticoid-enhanced transformation.

【 授权许可】

Unknown