Localization of a regulatory region on the 5′‐untranslated region of human hepatoma HepG2 γ‐glutamyltransferase mRNA and response to dexamethasone and antisense oligonucleotide treatment

FEBS Letters

Diederich, Marc¹  Wellman, Maria¹  Siest, Gérard¹ 

[1]Centre du Médicament, URA CNRS 597, 30, Rue Lionnois, F-54000 Nancy, France

关键词: γ-Glutamyltransferase;    5′-Untranslated region;    Transient expression;    Translational regulation;    Glucocorticoid hormone;    Hormone response element;    Antisense oligonucleotide;    Human hepatoma;    b;    base(s);    bp;    base pairs;    GGT;    γ-glutamyltransferase;    n;    nucleotide(s);    Tricine;    N-[2-hydroxy-1;    1-bis(hydroxymethyl)ethyl]-glycine;    5′UTR;    5′-untranslated region;   

DOI  :  10.1016/0014-5793(94)01293-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We are reporting the functional analysis of the 5′-untranslated region (5′UTR) of human hepatoma HepG2 γ-glutamyltransferase (GGT) mRNA. Transient expression of hybrid GGT-luciferase reporter gene mutants in HepG2 shows that this 5′UTR acts as a tissue-specific translational enhancer. A domain of 173 bases containing a steroid hormone response element (HRE) is responsible for the enhancing effect, which can be amplified by addition of dexamethasone at 10⁻⁶ M. Furthermore, the regulatory role of the 5′UTR is demonstrated by interaction with sense and antisense oligonucleotides.

【 授权许可】

Unknown