Stimulation of specific GTPase activity by vasopressin in isolated membranes from cultured rat hepatocytes

FEBS Letters

Brindley, David N.¹  Fain, John N.¹  Hawthorne, John N.¹  Pittner, Richard A.¹ 

[1] Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, Nottingham NG7 2UH, England

关键词: GTPase;    Guanine nucleotide binding protein;    Liver;    Phosphoinositide;    Vasopressin;   

DOI  :  10.1016/0014-5793(85)80118-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Membranes were isolated by isotonic homogenization and differential centrifugation from rat hepatocytes cultured overnight. The specific GTPase activity of the membranes was 1–1.3 pmol γ-labelled GTP hydrolysed/mg protein per min in the presence of 1.2 mM Na⁺, 2 mM EGTA, 1 mM ATP and 0.2 mM 5-adenylyl imidodiphosphate. Under these conditions there was a stimulation of specific GTPase activity of no more than 20% by 11–115 nM vasopressin. No effect of vasopressin was seen in the presence of 1.7 μM free Ca²⁺ or 100 mM Na⁺. The findings indicate that vasopressin is able to influence GTPase activity as well as accelerate phosphoinositide breakdown in rat hepatocytes.

【 授权许可】

Unknown