Fluorescent labelling of histone H3: effect on histone‐histone interaction and core particle assembly

FEBS Letters

Pretorius, Linda¹  Thompson, Patricia¹  von Holt, Claus¹  Lindsey, George G.¹ 

[1] UCT-CSIR Research Centre for Molecular Biology, Department of Biochemistry, University of Cape Town, Private Bag, Rondebosch 7700, Republic of South Africa

关键词: Histone H3;    Fluorescence;    Core particle;    Polyglutamic acid;    IAEDANS;    N-iodoacetyl-N-(5-sulpho-1-naphthyl)ethylenediamine;    H3-(Cys-IAEDANS);    histone H3 specifically labelled with IAEDANS at Cys 110;    H3-(IAEDANS);    histone H3 non-specifically labelled with IAEDANS;    H3-H3-(IAEDANS);    histone H3 Cys dimer non-specifically labelled with IAEDANS as the dimer;   

DOI  :  10.1016/0014-5793(85)80113-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Substitution of Cys 110 of chicken histone H3 with N-iodoacetyl-N ¹-(5-sulpho-1-naphthyl)ethylenediamine or iodoacetamide prevents octamer formation in 2 M NaCl but does not prevent polyglutamic acid-mediated core particle assembly.

【 授权许可】

Unknown