Folding and unfolding of the core particle DNA are processes faster than millisecond

FEBS Letters

Pouyet, J.¹  Burggraf, E.¹  Erard, M.¹ 

[1] Laboratoire de Biophysique, Institut de Biologie Moléculaire et Cellulaire, 15, rue Descartes, 67084 Strasbourg Cédex, France

关键词: Chromatin dynamics;    Core particle;    Dissociation;    Reassociation;    Circular dichroism;    Stopped-flow;   

DOI  :  10.1016/0014-5793(82)81070-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Core particle DNA unfolding and refolding are followed by stopped-flow circular dichroism technique. When core particles are dissociated in the stopped-flow cuvette, the high CD deviation corresponding to the dissociated state is reached in the first millisecond, which means that the dissociation process is completed within the dead time of the apparatus which is ∼1 ms. The same conclusion can be drawn when core particles are reassociated, since the low CD value, typical of the associated state, is immediately reached. Similarly histone release from chromatin is a very fast process. We also include some points of discussion about core particle assembly process.

【 授权许可】

Unknown