Activation of yeast plasma membrane ATPase by phorbol ester

FEBS Letters

Mazón, María J.¹  Portillo, Francisco¹ 

[1] Departamento de Bioquímica, Facultad de Medicina de la Universidad Autónoma and Instituto de Investigaciones Biomédicas del Consejo Superior de Investigaciones Científicas, C/Arzobispo Morcillo no. 4, 28029 Madrid, Spain

关键词: ATPase;    Plasma membrane;    (Yeast);    Phorbol ester;    Phosphorylation;   

DOI  :  10.1016/0014-5793(85)80050-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) to yeast cells produces a 2-fold activation of the plasma membrane ATPase. The activation is reversible and time- and dose-dependent. The activated enzyme shows an increased affinity for its substrate, ATP, and its optimum pH is shifted to a more alkaline range. These changes are similar to those observed in the reported activation by glucose. Upon incubation of yeast cells with ³²P_i incorporation of radioactivity in a membrane polypeptide of 105 kDa is observed after addition of either glucose or TPA.

【 授权许可】

Unknown