期刊论文详细信息
FEBS Letters | |
Activation of yeast plasma membrane ATPase by phorbol ester | |
Mazón, María J.1  Portillo, Francisco1  | |
[1] Departamento de Bioquímica, Facultad de Medicina de la Universidad Autónoma and Instituto de Investigaciones Biomédicas del Consejo Superior de Investigaciones Científicas, C/Arzobispo Morcillo no. 4, 28029 Madrid, Spain | |
关键词: ATPase; Plasma membrane; (Yeast); Phorbol ester; Phosphorylation; | |
DOI : 10.1016/0014-5793(85)80050-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) to yeast cells produces a 2-fold activation of the plasma membrane ATPase. The activation is reversible and time- and dose-dependent. The activated enzyme shows an increased affinity for its substrate, ATP, and its optimum pH is shifted to a more alkaline range. These changes are similar to those observed in the reported activation by glucose. Upon incubation of yeast cells with 32Pi incorporation of radioactivity in a membrane polypeptide of 105 kDa is observed after addition of either glucose or TPA.
【 授权许可】
Unknown
【 预 览 】
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