Binding of macrophages and phospholipid flip‐flop in supported lipid bilayers

FEBS Letters

Nakanishi, Mamoru²  Takahashi, Seizo¹  Matsumoto, Kazuko¹ 

[1] Department of Chemistry, Japan Women's University, Mejirodai, Bunkyo-ku, Tokyo 112, Japan;Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Supported bilayer;    Planar membrane;    Flip-flop;    Antibody subclass;    Macrophage;    Fc receptor;    DMPC;    dimyristoylphosphatidylcholine;    PBS;    phosphate-buffered saline;    TNPCap-DPPE;    trinitrophenylaminocaproylphosphatidylethanolamine;    FCS;    fetal calf serum;    NBDDPPE;    N (7-nitro-2;    1;    2-benzoxodiazol-4-yl)dipalmitoylphosphatidylethanolamine;   

DOI  :  10.1016/0014-5793(85)80044-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Subclass-specific antibody-dependent interactions (binding and triggering) between macrophages and supported lipid bilayers have been studied. Percentages of mouse macrophage binding (J774 cell line) to the lipid bilayers were dependent on mouse monoclonal IgG subclasses. The efficiencies were as follows: IgG1 = IgG2a > IgG2b > IgG3. Furthermore, macrophage triggering (spreading) was more efficient on IgG2a-or IgGl-coated lipid bilayers than on IgG2a, IgG3, or non-specific rabbit IgG. The present experiments show also that phospholipid molecules are able to flip-flop from one side of a supported planar bilayer membrane to the other with a half-life of 10 h–1 day at 25°C.

【 授权许可】

Unknown