Formation of lipid—protein bilayers by micropipette guided contact of two monolayers

FEBS Letters

Schindler, Hansgeorg¹  Schuerholz, Theo¹ 

[1] Biocenter, University of Basel, CH-4056 Basel, Switzerland

关键词: Membrane reconstitution;    Planar membrane;    Vesicle spreading;    Surface pressure;    Single channel;    Membrane tension;   

DOI  :  10.1016/0014-5793(83)80376-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bilayers of a few μm² area were formed from lipid-protein vesicles. First, two monolayers are generated from vesicles, which then are brought into local contact by use of a micro-pipette to form a bilayer. Membrane channels of Matrix—Protein and of Colicin A exhibit normal properties in these bilayers. Apart from avoiding solvents completely and of using characterized vesicles as starting materials there are two main virtues to this strategy: (1) Current resolution is very high, a few tenths of a pA at 150 μs time resolution; and (2) transport processes can be studied in dependence of surface pressure between 28 and 48 mN/m.

【 授权许可】

Unknown