13C‐NMR reveals glycerol as an unexpected major metabolite of the protozoan parasite Trichomonas vaginalis

FEBS Letters

Williams, J.²  Chapman, A.¹  Lloyd, D.¹  Linstead, D.J.² 

[1] Department of Microbiology, University College, PO Box 78. Cardiff CF1 1XL, Wales UK;Departments of Physical Chemistry and Biochemical Parasitology, Wellcome Research Laboratories, Beckenham BR3 3BS, England

关键词: Trichomonas vaginalis;    Glucose metabolism;    13C-NMR;    Metabolite formation;    Glycerol;    [13C]Glucose;   

DOI  :  10.1016/0014-5793(85)80026-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

¹³C-NMR has been used to study the kinetics of the formation of metabolites from [l-¹³C]glucose in intact cells of Trichomonas vaginalis during anaerobic incubation. As well as the expected metabolites lactate and acetate, this technique revealed glycerol as an additional major product, present in amounts equimolar with acetate. The formation of glycerol is readily explained in terms of the need to maintain redox balance. This protozoan now joins the small group of organisms which are known to produce glycerol as a result of normal metabolic activities.

【 授权许可】

Unknown