Inhibition of poly(ADP‐ribose) polymerase causes increased DNA strand breaks without decreasing strand rejoining in alkylated HeLa cells

FEBS Letters

Ebisuzaki, Kaney¹  Moran, Michael F.¹ 

[1] Cancer Research Laboratory and Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5B7, Canada

关键词: 3AB;    3-aminobenzamide;    araC;    cytosine-β-D-arabinofuranoside;    DMS;    dimethyl sulfate;    PBS;    phosphate-buffered saline;    AP;    apurinic/apyrimidinic;   

DOI  :  10.1016/0014-5793(85)81300-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of alkylated HeLa cells with 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase, increased the number of DNA strand breaks but did not affect the rate of strand rejoining. This suggests that an increase in DNA incision, not a decrease in ligation, results from the inhibition ofpoly(ADP-ribose) polymerase in cells recovering from DNA damaged by alkylating agents. Poly(ADP-ribose) DNA strand break DNA repair

【 授权许可】

Unknown