Presence of helodermin‐like peptides of the VIP‐secretin family in mammalian salivary glands and saliva

FEBS Letters

de Neef, Philippe²  Vandermeers, André²  Vandermeers-Piret, Marie-Claire²  de Graef, Jacques¹  Christophe, Jean²  Meuris, Sylvian³  Svoboda, Michal²  Yanaihara, Chizuko⁴  Woussen-Colle, Marie-Claire¹  Yanaihara, Noboru⁴  Robberecht, Patrick² 

[1] Laboratory of Experimental Surgery L. Delayers, Hôpital Universitaire Saint-Pierre, B-1000 Brussels, Belgium;Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Bd de Waterloo 115, USA;Laboratory of Gynecology and Obstetrics L. Delayers, Hôpital Universitaire Saint-Pierre, B-1000 Brussels, Belgium;Laboratory of Bioorganic Chemistry, Shizuoka College of Pharmacy, Shizuoka, Shizuoka 422, Japan

关键词: Helodermin;    Gila monster venom;    Secretin/VIP peptide family;    Mammalian salivary gland;    Saliva;   

DOI  :  10.1016/0014-5793(85)80445-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Helodermin is a biologically active peptide isolated from the venom of the Gila monster lizard (Heloderma suspectum) whose structure is related to that of vasoactive intestinal peptide and secretin. Using a specific radioimmunoassay based on antisera prepared by immunizing rabbits with natural helodermin, we demonstrated the presence of helodermin-like material in mammalian salivary glands, including parotid, submaxillary and sublingual glands from rat and dog, and parotid and submaxillary glands from man. All helodermin-like materials had an apparent molecular mass of 4–12 kDa. Dog saliva, collected after pilocarpine stimulation, revealed similar immunoreactivity with a major component around 6 kDa.

【 授权许可】

Unknown