Cytoplasmic pH, a key determinant of growth factor‐induced DNA synthesis in quiescent fibroblasts

FEBS Letters

Pouysségur, J.¹  Franchi, A.¹  L'Allemain, G.¹  Paris, S.¹ 

[1] Centre de Biochimie, CNRS, Université de Nice, Parc Valrose, 06034 Nice, France

关键词: Na+/H+ antiporter;    pHi;    Fibroblast mutant;    DNA synthesis;    Growth factor;   

DOI  :  10.1016/0014-5793(85)80439-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In response to growth factors, quiescent fibroblast mutants lacking Na⁺/H⁺ exchange activity fail to elevate their cytoplasmic pH (pH_i) and to reinitiate DNA synthesis at neutral and acidic pH_o. A pH_i threshold of ~7.2 exists, below which growth factors cannot set in motion the G₀ to S phase transition. Restoration of the pH_i defect in mutant cells restores the wild-type phenotype. These findings, combined with the properties of another class of mutants able to grow at very low pH_o, demonstrate that pH_i modulated by growth factor activation of the Na⁺/H⁺ antiporter, plays a determinant role in growth control.

【 授权许可】

Unknown