Rapid exchange of histones H2A and H2B in sea urchin embryo chromatin

FEBS Letters

Schwager, S.¹  von Holt, C.¹  Retief, J.D.¹  de Groot, P.¹ 

[1] UCT-CSIR Research Centre for Molecular Biology, Department of Biochemistry, University of Cape Town, Private Bag Rondebosch 7700, Republic of South Africa

关键词: Sea urchin embryo;    Nucleosome;    Chromatin;    Histone;   

DOI  :  10.1016/0014-5793(85)81045-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chromatin from sea urchin blastula and gastrula was partially digested with micrococcal nuclease and separated electrophoretically in two dimensions into various nucleosome fractions and their component histones. After labelling of the embryos with radioactive lysine the various isohistones of the H2A and H2B group in all nucleosome fractions had incorporated approximately twice as much the radioactivity as the histones H2 and H4. The results are interpreted in terms of a labile nucleosome structure during transcription.

【 授权许可】

Unknown