A small phospholipase inhibitory factor released by cultured cell lines

FEBS Letters

Salmon, John¹  Wood, John N.¹  Rhodes, John¹  Coote, Paul R.¹ 

[1] Department of Experimental Immunobiology and Department of Prostaglandin Research, The Wellcome Research Laboratories, Beckenham, Kent, England

关键词: Arachidonic acid;    Prostaglandin;    Phospholipase;    Macrophage;   

DOI  :  10.1016/0014-5793(85)81023-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cells of the mouse macrophage-like cell line RAW₂₆₄ release a dialysable inhibitor of phospholipase activity into their culture medium. This inhibitor can be detected in saline solution, Hanks solution and a variety of tissue culture media in the presence or absence of serum. The inhibitor is stable at 4°C, unaffected by trypsin, nucleases, or boiling, and partially extractable with chloroform/methanol. The release of both arachidonic acid and prostaglandins from mouse macrophages or human monocytes is inhibited by this material. A variety of other cell types release the inhibitor, which is effective against stimulation of arachidonic acid release from cultured macrophages by zymosan, serum, immune complexes and the calcium ionophore A23187.

【 授权许可】

Unknown