Uptake of rat and human α2‐macroglobulin‐trypsin complexes into rat and human cells

FEBS Letters

Davidsen, Ole¹  Sonne, Ole¹  Sottrup-Jensen, Lars²  Gliemann, Jørgen¹ 

[1] Institute of Physiology, University of Aarhus, DK-8000 Aarhus C, Denmark;Institute of Molecular Biology, University of Aarhus, DK-8000 Aarhus C, Denmark

关键词: α2;    Macroglobulin;    Adipocyte;    Hepatocyte;    Species difference;   

DOI  :  10.1016/0014-5793(85)80401-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Uptake of rat and human α₂-macroglobulin-trypsin complexes was measured in rat hepatocytes, rat and human adipocytes and human fibroblasts. Uptake and degradation of ¹²⁵I-labelled rat complex were about one-third of that of the human complex in the various isolated cell types. In rat hepatocytes, the apparent K _m for cell association of the rat complex was about 16 nM as compared to about 6 nM for the human complex. The V _max values were similar, about 1 × 10⁴ molecules · cell⁻¹ · min⁻¹. Thus, rat α₂-macroglobulin (an acute-phase protein) complexed with trypsin follows the same pathways of uptake as the human homologue, although with a somewhat lower affinity for the uptake system.

【 授权许可】

Unknown