Cloning of two β‐tubulin related genes in tetrahymena pyriformis

FEBS Letters

Barahona, Isabel¹  Jacquet, Michel²  Galego, Lisete¹  Rodrigues-Pousada, Claudina¹ 

[1] Departamento de Microbiologia, Institute Gulbenkian de Ciência, Apartado 14, 2781 Oeiras Codex, Portugal;Laboratoire de Biologie Expérimentale Bâtiment 400, Université Paris Sud, 91405 Orsay Cedex, France

关键词: β-Tubulin;    Gene organization;    Tetrahymena pyriformis;   

DOI  :  10.1016/0014-5793(85)80377-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Genomic clones containing β-tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin-related genes has been established by cross-hybridization with a cDNA containing a complete β-tubulin coding region from Chlamydomonas and by the positive hybrid-selected translation assay. The respective tubulin-related genes, βTT₁ and βTT₂, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β-tubulin-related genes are physically linked and clustered in the genome.

【 授权许可】

Unknown