Hydrophobic properties of Tetrahymena calmodulin related to the phosphodiesterase activity

FEBS Letters

Naka, Michiko¹  Hidaka, Hiroyoshi¹  Nozawa, Yoshinori²  Inagaki, Masaki¹ 

[1] Department of Pharmacology, Mie University of Medicine, Edobashi, Tsu 514, Japan;Department of Biochemistry, Gifu University of Medicine, Gifu 500, Japan

关键词: Tetrahymena pyriformis;    Calmodulin;    Ca2+-CaM-dependent phosphodiesterase;    N-Phenyl-l-naphthylamine;    Hydrophobicity;    Calcium;    CaM;    Calmodulin;    NPN;    N-phenyl-1-naphthylamine;    EGTA;    ethylene glycol bis (β-aminoethyl ether)-N;    N;    N;    N-tetraacetic acid;   

DOI  :  10.1016/0014-5793(83)80344-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined hydrophobic properties of Tetrahymena CaM using the uncharged probe, n-phenyl-1-naphthylamine (NPN) fluorescence. The maximal flurorescence intensity of Tetrahymena calmodulin (CaM) is < 1/12 of the bovine brain CaM. In the phosphodiesterase activation, the potency of Tetrahymena CaM, which was represented by reciprocals of the quantity of CaM required for half-maximal activation of enzyme was 22.7% respectively, of that of the bovine brain CaM. Here, Tetrahymena CaM had less hydrophobic groups exposed in the presence of Ca²⁺. Then Ca²⁺-CaM dependent enzymes require much amount of Tetrahymena CaM, comparing with the bovine brain CaM.

【 授权许可】

Unknown