Comparison of genomes of closely related phages ø29, ø15 and PZA using a rapid method of parallel physical mapping

FEBS Letters

Zadražil, Stanislav¹  Hostomský, Zdeněk¹  Pačes, Václav¹ 

[1] Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Flemingovo nám.2,166 37 Prague 6, Czechoslovakia

关键词: Bacillus subtilis phage;    DBM paper hybridization;    Restriction analysis;    Physical mapping;    DBM;    diazobenzyloxymethyl;    bp;    base pairs;    kb;    kilobase pairs;   

DOI  :  10.1016/0014-5793(85)80887-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The DNAs of phages ø29, ø15 and PZA of Bacillus subtilis were analysed with restriction enzymes EcoRI, HpaI and HindIII. A method was used which permits parallel physical mapping of all three phages, from both ends of their linear genomes. The method is based on transfer of partially digested DNA to DBM paper and sequential hybridization with labelled terminal fragments. It follows from the comparison of the physical maps that phages ø29, ø15 and PZA are closely related and that they probably have arisen from a common ancestor by accumulation of point mutations.

【 授权许可】

Unknown