Final steps of the maturation of Omp F, a major protein from the outer membrane of Escherichia coli

FEBS Letters

Barbas, J.A.¹  Vázquez, D.¹  Rodríguez-Tébar, A.¹ 

[1] Instituto de Biología Molecular, CSIC-UAM, Facultad de Ciencias, Canto Blanca, 28049 Madrid, Spain

关键词: E. coli;    Outer membrane;    Porin protein;    Omp F;    Membrane biogenesis;    Chemical cross-linking;   

DOI  :  10.1016/0014-5793(85)80877-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pulse-labelling experiments with E. coli cells allowed us to follow the incorporation of de novo proteins into the outer membrane of the cell envelope. Labelled membrane samples containing increasingly different levels of newly synthesized Omp F protein were subjected to chemical cross-linking with a bifunctional cleavable reagent in order to investigate the process of trimer formation of the protein. From the results obtained, we conclude that the formation of functional Omp F trimers is substantially delayed to, and can be distinguished from, the incorporation of Omp F monomers to the outer membrane.

【 授权许可】

Unknown