Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid

FEBS Letters

Dinh, Daniéle Pham²  Alliel, Patrick M.²  Galibert, Francis¹  Joliès, Pierre²  Nussbaum, Jean-Louis³  d'Auriol, Luc¹  Dautigny, André² 

[1] Laboratoire d'Hématologie Expérimentale, Centre Hayem, Hôpital Saint-Louis, 2 place du Docteur Fournier, 75475 Paris Cedex 10, France;Laboratoire des Protéines, Université de Paris V, 45, rue des Saints-Peres, 75270 Paris Cedex 06, France;Institut de Neurochimie du CNRS, Laboratoire de Chimie Biologique, 67084 Strasbourg Cedex, France

关键词: Rat brain;    Myelin;    Proteolipid;    Oligonucleotide probe;    cDNA sequence;   

DOI  :  10.1016/0014-5793(85)80869-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.

【 授权许可】

Unknown