FEBS Letters | |
Cloning of the β‐phycocyanin gene from Anacystis nidulans | |
Lind, Lisbet K.2  Lönneborg, Anders3  Kalla, S.Roger2  Öquist, Gunnar3  Gustafsson, Petter1  | |
[1]Departments of Microbiology, University of Umeå, S-901 87 Urneå, Sweden | |
[2]Plant Physiology, University of Umeå, S-901 87 Urneå, Sweden | |
关键词: Cyanobacteria; Anacystis nidulans; Phycobilisome; β-Phycocyanin; Photosynthesis gene; DNA sequence; Light-harvesting antenna; | |
DOI : 10.1016/0014-5793(85)80868-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The β-phycocyanin gene, pcyB, of Anacystis nidulans was isolated from an Escherichia coli λ-phage bank by the use of synthetic oligonucleotides derived from the 170 amino acid sequence of the β-phycocyanin protein. Two positive, overlapping λ-clones were found. Partial DNA sequencing of one of the clones gave a deduced amino acid sequence which was in full agreement with a portion of the published sequence of A. nidulans β-phycocyanin. A comparison with the published DNA sequence for β-phycocyanin from Agmenellum quadruplicatum shows a DNA sequence homology of 70.4% over the sequenced region.
【 授权许可】
Unknown
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