Cloning of the β‐phycocyanin gene from Anacystis nidulans

FEBS Letters

Lind, Lisbet K.²  Lönneborg, Anders³  Kalla, S.Roger²  Öquist, Gunnar³  Gustafsson, Petter¹ 

[1] Departments of Microbiology, University of Umeå, S-901 87 Urneå, Sweden;Plant Physiology, University of Umeå, S-901 87 Urneå, Sweden

关键词: Cyanobacteria;    Anacystis nidulans;    Phycobilisome;    β-Phycocyanin;    Photosynthesis gene;    DNA sequence;    Light-harvesting antenna;   

DOI  :  10.1016/0014-5793(85)80868-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The β-phycocyanin gene, pcyB, of Anacystis nidulans was isolated from an Escherichia coli λ-phage bank by the use of synthetic oligonucleotides derived from the 170 amino acid sequence of the β-phycocyanin protein. Two positive, overlapping λ-clones were found. Partial DNA sequencing of one of the clones gave a deduced amino acid sequence which was in full agreement with a portion of the published sequence of A. nidulans β-phycocyanin. A comparison with the published DNA sequence for β-phycocyanin from Agmenellum quadruplicatum shows a DNA sequence homology of 70.4% over the sequenced region.

【 授权许可】

Unknown