Effect of ascorbic acid on the production of singlet oxygen by purified human myeloperoxidase

FEBS Letters

Kanofsky, Jeffrey R.²  Tauber, Alfred I.³  Wright, Jonathan¹ 

[1] Loyola University, Stritch School of Medicine, Maywood, IL 60153 USA;Departments of Medicine, Edward Hines, Jr., VA Hospital, Hines, IL 60141, USA;The William B. Castle Hematology Research Laboratory, Boston City Hospital, USA

关键词: Ascorbic acid;    Myeloperoxidase;    Singlet oxygen;   

DOI  :  10.1016/0014-5793(85)81263-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously studied purified human myeloperoxidase-hydrogen peroxide-halide ion systems as models of possible singlet oxygen production by granulocytes. While myeloperoxidase could efficiently produce singlet oxygen, the yield of singlet oxygen at a physiological pH with Cl⁻ was very small due to enzyme inactivation. In that Bolscher et al. [(1984) Biochim. Biophys. Acta 784, 189-191] observed that micromolar concentrations of ascorbic acid prevented inactivation of myeloperoxidase and increased the production of hypochlorous acid, we examined whether ascorbic acid would augment singlet oxygen production by the myeloperoxidase-hydrogen peroxide-halide ion systems. Ascorbic acid, however, fails to increase the singlet oxygen yield, suggesting that it does not augment singlet oxygen production in the intact granulocyte by a myeloperoxidase-dependent mechanism.

【 授权许可】

Unknown