The effect of gramicidin on ATP synthesis in pea chloroplasts: two modes of phosphorylation

FEBS Letters

Kuz'mina, V.P.¹  Yaguzhinsky, L.S.¹  Red'ko, T.P.¹  Opanasenko, V.K.¹ 

[1] Soil Science and Photosynthesis Institute of the USSR Academy of Sciences, Pushchino and A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Thylakoid membrane;    Photophosphorylotion;    Protein uptake;    Proton-binding domain;    Photosystem II;    Electron flow;    (Pea chloroplast);    PS II;    photosystem II;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    MV;    methyl viologen;    PMS;    phenazine methosulfate;   

DOI  :  10.1016/0014-5793(85)81254-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of gramicidin on the phosphorylation rate, electron flow and light-induced H²⁺ uptake (ΔH⁺) in chloroplasts with methyl viologen (MV) or phenazine methosulfate (PMS) added has been studied. In the presence of MV low concentrations of gramicidin (~10⁻⁹ M) were shown to inhibit the phosphorylation rate up to 80–90% of the initial value, without changing either the electron transport rate or ΔH⁺ value. Two components of phosphorylation have been identified in the presence of PMS - the first is DCMU-sensitive, which was suppressed by low gramicidin concentrations (< 10⁻⁹ M) and the second - DCMU-insensitive component, which was suppressed by high gramicidin concentrations (~10⁻⁷ M) exclusively, the high gramicidin concentration inducing the ΔH⁺ value fo fall.

【 授权许可】

Unknown