Chemically modified low density lipoproteins as inducers of enzyme release from macrophages

FEBS Letters

Hartung, Hans-Peter²  Kladetzky, Rudolf G.¹  Hennerici, Michael² 

[1] Department of Medicine, University of Düsseldorf, Moorenstr. 5, D-4000 Düsseldorf 1, FRG;Department of Neurology, University of Düsseldorf, Moorenstr. 5, D-4000 Düsseldorf 1, FRG

关键词: Macrophage receptor;    Acetylated LDL;    Malondialdehyde-altered LDL;    Enzyme release;    Atherosclerosis;   

DOI  :  10.1016/0014-5793(85)80710-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Macrophages carry receptors on their surface for acetylated low density lipoprotein (ac-LDL). Receptor-mediated endocytosis of ac-LDL is followed by intracellular cholesterol accumulation. We investigated whether occupation of these binding sites evokes the release of hydrolytic enzymes from mouse peritoneal macrophages cultured for up to 48 h. ac-LDL at concentrations ranging from 25–250 was noted to promote in a dose-dependent fashion secretion of the neutral proteinase elastase (EC 3.4.21.37) and the lysosomal acid hydrolases N-acetyl-β-glucosaminidase (EC 3.2.1.30), β-glucuronidase (EC 3.2.1.31), β-galactosidase (EC 3.2.1.23), α-mannosidase (EC 3.2.1.24) and cathepsin D (EC 3.4.23.5). This stimulatory effect was non-cytotoxic. LDL modified by treatment with malondialdehyde was also capable of augmenting enzyme liberation into culture supernates. These findings may have implications for some aspects of the atherosclerotic process.

【 授权许可】

Unknown