In vivo and in vitro catabolism of native and biologically modified LDL

FEBS Letters

van Berkel, Theo J.C.¹  Havekes, Louis²  Nagelkerke, J.Fred¹  van Hinsbergh, Victor W.M.² 

[1] Biochemistry I, Medical Faculty, Erasmus University, PO Box 1738, 3000 DR Rotterdam The Netherlands;Gaubius Institute, TNO, Herenstraat 5, 2313 AD Leiden, The Netherlands

关键词: Human LDL;    Biologically modified LDL;    Acetylated LDL;    Human umbilical-vein endothelial cell;    Rat liver endothelial cell;    Rat liver Kupffer cell;    BSA;    bovine serum albumin;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(84)80477-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of human low density lipoprotein (LDL) at 37°C in the presence of human umbilical-vein endothelial cells (EC) causes a time-dependent shift in the charge and density of LDL. After intravenous injection into rats, native LDL is merely cleared from the circulation by Kupffer cells while EC-modified LDL is rapidly cleared by endothelial liver cells. The uptake of native LDL by Kupffer cells and EC-modified LDL by endothelial cells in vivo can be explained by the presence of two different specific receptors on these cell types. It is concluded that the liver endothelial cells form an important protection against a possible atherogenic action of EC-modified LDL.

【 授权许可】

Unknown