Identification of glycine spin systems in 1H NMR spectra of proteins using multiple quantum coherences

FEBS Letters

Boyd, Jonathan²  Redfield, Christina¹  Dobson, Christopher M.¹ 

[1] Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QR, England;Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QR, England

关键词: 1H NMR;    Assignment;    Glycine;    Human lysozyme;    Multiple-quantum NMR;    DQF;    double-quantum filtered;    TQF;    triple-quantum filtered;   

DOI  :  10.1016/0014-5793(85)81334-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Double-quantum filtered COSY and triple-quantum filtered COSY techniques have been compared for the tripeptide Gly-Tyr-Gly and for human lysozyme. The insertion of a triple-quantum filter in the COSY experiment leads to dramatic spectral simplification in the fingerprint region of the spectrum and permits the specific identification of glycine spin systems in the complex ¹H NMR spectra of proteins. The assignment of these peaks to glycine H^α can be confirmed using 2D double-quantum correlated spectroscopy.

【 授权许可】

Unknown