Nuclear Overhauser effects and the assignment of the proton NMR spectra of proteins

FEBS Letters

Redfield, Christina¹  Dobson, Christopher M.¹  Howarth, Martin A.¹ 

[1] Inorganic Chemistry Laboratory, South Parks Road, Oxford 0X1 3QR, England

关键词: 1H NMR;    Lysozyme;    NOE;    Assignment;    NOE;    nuclear Overhauser enhancement;    Iysozyme;    hen egg-white Iysozyme (EC 3.2.1.17);   

DOI  :  10.1016/0014-5793(84)81185-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A systematic procedure is outlined to reveal assignments in the ¹H NMR spectrum of a protein that are consistent with a limited set of nuclear Overhauser effect data and with specific assumptions. The results of applying this to a group of resonances in the spectrum of hen lysozyme, most of which were previously assigned by independent methods, are described. The number of possible assignment schemes obtained depends on the distance constraints chosen for the Overhauser effect data, and the investigation of this dependence is proposed as a method of assessing the confidence that can be placed in individual assignments obtained in this way.

【 授权许可】

Unknown