C1q binding to mitochondria: a possible artefact?

FEBS Letters

Easterbrook-Smith, Simon B.¹  Comis, Alfio¹ 

[1] Department of Biochemistry, University of Sydney, Sydney, NSW 2006, Australia

关键词: Clq;    Mitochondria;    Nucleic acid;    Nuclease;    Cyclohexane-1;    2-dione;   

DOI  :  10.1016/0014-5793(85)80749-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitochondrial preparations, obtained from human tonsils and from rat spleen, liver, heart, and kidney tissues, bound [¹²⁵I]Clq with affinities of 10⁷–10⁸ M⁻¹. The binding of Clq was not affected by treatment of the mitochondrial preparations with pronase, trypsin, or phospholipase D, but it was lowered 5–6-fold following treatment of the mitochondria with DNase and RNase. Binding of Clq to mitochondrial preparations was also greatly diminished by limited chemical modification of Clq with cyclohexane-1,2-dione. It is suggested that the reported binding of Clq to mitochondria may have arisen from the protein binding to DNA and/or RNA contaminating the mitochondrial preparations.

【 授权许可】

Unknown