Electron transport driven phosphorylation catalyzed by proteoliposomes containing hydrogenase, fumarate reductase and ATP synthase

FEBS Letters

Bokranz, Martin¹  Kröger, Achim¹  Graf, Margret¹  Böcher, Reinhard¹  Friedl, Peter² 

[1] Fachbereich Biologie / Mikrobiologie, Philipps-Universität Marburg, Lahnberge, 3550 Marburg, FRG;Gesellschaft für Biotechnologische Forschung, Braunschweig-Stöckheim, FRG

关键词: Electron transport;    Phoshorylation;    Liposome;    Fumarate;    reductase;    Hydrogenase;    DCCD;    N;    N'-dicyclohexylcarbodi-imide;    DMN;    2;    3-dimethyl-1;    4-naphthoquinone;    DMNH2;    reduced DMN;    FCCP;    carbonylcyanide-4-tri-fluoromethoxyphenylhydrazone;    Mops;    3-(N-morpho-lino) propanesulfonate;   

DOI  :  10.1016/0014-5793(85)80662-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Liposomes containing the hydrogenase complex and the fumarate reductase complex isolated from Wolinella (formerly Vibrio) succinogenes, together with vitamin K₁ catalyzed the electron transport from H₂ to fumarate. With the fumarate reductase complex present in excess the activity of electron transport was close to that of the hydrogenase complex. Liposomes containing an ATP synthase in addition to the electron transport components catalyzed the phosphorylation of ADP driven by the electron transport.

【 授权许可】

Unknown