Incorporation of isoleucine into protein by a soluble fraction from spermatozoa

FEBS Letters

Hashizume, Shuichi¹ 

[1] Morinaga Institute of Biological Science, Shimosueyoshi 2-1-1, Tswwni-ku, Yokohama, 230 Japan

关键词: Isoleucine;    Spermatozoa;    Incorporation;    105 000 × g supernatant;   

DOI  :  10.1016/0014-5793(85)80649-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A heat-labile, non-dialyzable factor(s) in soluble fractions from porcine, bull, rabbit and cock spermatozoa was found to incorporate the radioactivity of [¹⁴C]isoleucine into a 95°C CCl₃COOH-insoluble fraction. The incorporation required ATP, Mg²⁺, casein and 2-mercaptoethanol. Trypsin and a-chymotrypsin inhibited the incorporation, while RNase A and DNase I did not. A mixture of 19 amino acids other than isoleucine had no effect on the incorporation. The reaction product was identified as protein. The incorporated moiety was the isoleucyi moiety of isoleucine and it retained a free α-amino group in the product protein. Some other characteristics of this incorporation are also described.

【 授权许可】

Unknown