Sodium‐dependent isoleucine transport in the methanogenic archaebacterium Methanococcus voltae

FEBS Letters

Bird, Sharon E.¹  Sprott, G.Dennis¹  Jarrell, Ken F.¹ 

[1] Division of Biological Sciences, National Research Council of Canada, Ottawa K1A OR6, Canada

关键词: Sodium-dependent transport;    Isoleucine;    Methanococcus voltae;   

DOI  :  10.1016/0014-5793(84)80112-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Methanococcus voltae possesses a Na⁺-dependent transport system for isoleucine which requires for optimum rates a CO₂/H₂ atmosphere. The K _m for the system is 4.5 μM with a V _max of 1.5 nmol·min⁻¹·mg dry wt⁻¹. Approximately 75% of the label can be released from the cell pool following short-term experiments with gradients of isoleucine reaching 100 (in/out). Transport is inhibited by ionophores and N-ethyl maleimide. Only valine and leucine effectively compete with isoleucine for transport.

【 授权许可】

Unknown