FEBS Letters | |
Uric acid and allantoin uptake by Bacillus fastidiosus spores | |
Ellar, David J.1  Salas, Jose Antonio1  | |
[1] Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, England | |
关键词: Uric acid; Allantoin; Bacillus; Spore; Germination; Differentiation; | |
DOI : 10.1016/0014-5793(85)80788-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Uric acid enters Bacillus fasitidiosus spores by a constitutive carrier-mediated mechanism. The extent of uptake was proportional to the external uric acid concentration up to the limit of solubility. Most of the uric acid taken up after 2 min of incubation was not exchangeable with cold uric acid, suggesting that the uric acid was being quickly metabolized. Allantoin (an uric acid degradation product) was not incorporated by spores unles they were triggered to germinate with uric acid and the induced by allantoin. The induction of this uptake system was inhibited by chloramphenicol. The inability of spores to germinate in the sporulation medium was found to be due to the high pH of the sporulation medium after growth and sporulation.
【 授权许可】
Unknown
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