Induction of drug metabolizing enzymes in human liver cell line Hep G2

FEBS Letters

Adams, D.J.¹  Wolf, C.R.¹  Dawson, J.R.² 

[1] Imperial Cancer Research Fund, Medical Oncology Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland;University Department of Biochemistry, Edinburgh, Edinburgh EH4 2XU, Scotland

关键词: Cytochrome;    P-450;    Hep G2;    Cell line;    Drug metabolism;    Enzyme induction;    UDP-glucoronosyltransferase;   

DOI  :  10.1016/0014-5793(85)80780-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human cytochrome P-450, UDP-glucuronosyltransferase and sulphotransferase activities have been measured in the cell line Hep G2 following treatment of cells with 3-methylcholanthrene or phenobarbital. 3-Methylcholanthrene treatment caused a 20–30-fold increase in the O-deethylation of 7-ethoxycoumarin. The glucuronidation and sulphation of the product 7-hydroxycoumarin were increased 36 and 7 fold, respectively. In comparison, phenobarbital treatment did not increase these activities significantly. However, phenobarbital-inducible proteins were identified on ‘Western blots’ using antibodies to a rat liver phenobarbital inducible P-450 form. The molecular masses of the proteins did not coincide with those expected for cytochromes P-450. However, characteristic of P-450 forms, the synthesis of these proteins was suppressed by 3-methylcholanthrene treatment. The Hep G2 cell line represents a potentially useful model for studying the regulation of human P-450 genes.

【 授权许可】

Unknown