Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells

International Journal of Molecular Sciences

Keyong Xu¹  Feng Liu²  Benguo Liu³  Han Gao³ 

[1] College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China; E-Mail:;The Key Laboratory of Chemical Biology, Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Guangdong 518055, China; E-Mail:;School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China; E-Mails:

关键词: apoptosis;    cell cycle;    Hep G2;    4′;    7-diacetoxyapigenin;   

DOI  :  10.3390/ijms11051991

来源: mdpi

【 摘 要 】

In this study, 4′,7-diacetoxyapigenin [4-(7-acetoxy-5-hydroxy-4-oxo-4H-chromen-2-yl) phenyl acetate] was synthesized for the first time. Its chemical structure was identified by UV, ESI-MS, ¹H and ¹³C-NMR. It could inhibit the proliferation of Hep G2 cells in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treatment by 4′,7-diacetoxyapigenin, phosphatidylserine of Hep G2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-FITC and PI staining. It was concluded that 4′,7-diacetoxyapigenin not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.