Insulin processing and action in adipocytes: evidence for generation of insulin‐containing vesicles by leupeptin and monensin

FEBS Letters

Flint, David J.²  Mayer, R.John¹  Jeffrey, Ian W.¹  Vernon, Richard G.² 

[1] Department of Biochemistry, University of Nottingham Medical School, Clifton Boulevard, Nottingham NG7 2UH, England;The Hannah Research Institute, Ayr KA6 5HL, Scotland

关键词: Insulin processing;    Adipocyte;    Leupeptin;    Monensin;    Glucose transport;    Acetyl-CoA carboxylase;   

DOI  :  10.1016/0014-5793(85)80352-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of adipocytes with ¹²⁵I-insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of ¹²⁵I-insulin (modal density about 1.20 ) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surface. The fractions comprising this peak contained vesicles, probably originating from the Golgi apparatus, and dit not appear to be contaminated with lysosomes, mitochondria or plasma membrane. Entrapment of insulin in these vesicles per se did not prevent the activation of glucose transport, acetyl-CoA carboxylase or pyruvate dehydrogenase by insulin.

【 授权许可】

Unknown