Requirement for a reduced sulphydryl entity in the protection of molybdate‐stabilized estrogen receptor

FEBS Letters

Ratajczak, T.¹  Hähnel, R.¹  Samec, A.M.¹ 

[1] University of Western Australia, Department of Obstetrics and Gynaecology, King Edward Memorial Hospital for Women, Subiaco, WA 6008, Australia

关键词: Estrogen;    Receptor Stability;    Molybdate;    Leupeptin;    Dithiothreitol;   

DOI  :  10.1016/0014-5793(82)81076-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The separate and combined effects of molybdate and dithiothreitol on the stability of human uterine 9 S estrogen receptor were studied. Maximal, short-term, protection of the 9 S estrogen receptor was achieved by the joint inclusion of both stabilizing agents in cytosol buffers. This molybdate—dithiothreitol-mediated stability was dependent on reducing agent concentration inferring sulphydryl involvement in 9 S receptor protection by molybdate. The study also showed that molybdate—dithiothreitol could not prevent the gradual decay of the 9 S estrogen receptor to the 4 S form in cytosols stored at 4°C over prolonged periods.

【 授权许可】

Unknown