Proenzyme to urokinase‐type plasminogen activator in the mouse in vivo

FEBS Letters

Danø, K.¹  Nielsen, L.S.¹  Skriver, L.¹  Kielberg, V.¹  Andreasen, P.A.¹  Grøndahl-Hansen, J.¹ 

[1]Finsen Laboratory, Finsen Institute, Strandboulevarden 49, and Laboratory of Tumor Biology, Institute of Pathology, University of Copenhagen, Frederik d. V's Vej 11, 2100 Copenhagen Ø, Denmark

关键词: Plasminogen activator;    Urokinase-type;    Proenzyme;    Immunoblotting;    u-PA;    urokinase-type plasminogen activator;    t-PA;    tissue-type plasminogen activator;    TBS-T;    0.05 M Tris-HCl (pH 7.4);    0.15 M NaCl;    1% () Triton X-100;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(85)80350-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated whether urokinase-type plasminogen activator (u-PA) is present in the mouse in vivo as the proenzyme or as the active enzyme. u-PA in extracts of various murine tissues was of a one-polypeptide chain form with an electrophoretic mobility indistinguishable from purified proenzyme (pro-u-PA), as demonstrated by SDS-polyacrylamide gel electrophoresis under recucing conditions followed by immunoblotting. No 2-chain u-PA was detected in any of the extracts (detection limit 10% of that of one-chain u-PA). In bladder urine more than half of the u-PA was of the one-chain form. Together with previous immunocytochemical studies of the normal murine tissues and studies of the Lewis lung carcinoma, the present results indicate that in these tissues the one-chain proenzyme is the predominant form of u-PA in intracellular stores and for the first time demonstrates that at least in some cases the one-chain form constitutes a sizeable fraction of the u-AP in extracellular fluids in the intact organism.

【 授权许可】

Unknown