Propeptide of the metalloprotease of Brevibacillus brevis 7882 is a strong inhibitor of the mature enzyme

FEBS Letters

Serkina, Anna V.¹  Shevelev, Alexei B.¹  Gorozhankina, Tatiana F.¹  Chestukhina, Galina G.¹ 

[1] V.M. Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms (GNII Genetika), 1st Dorozhny, 1, Moscow, 113545, Russia

关键词: Proenzyme;    Tight-binding inhibition;    Brevibacillus brevis;    Dnp;    dinitrophenyl moiety;    FPLC;    fast protein liquid chromatography;    Npr;    neutral protease (metalloendopeptidase);    PAGE;    polyacrylamide gel electrophoresis;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/S0014-5793(99)00791-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A metalloprotease gene of Brevibacillus brevis (npr) was expressed in Escherichia coli in a soluble form as native Npr precursor. A significant fraction of the precursor was spontaneously processed, producing the N-terminal propeptide and the mature enzyme. A strong inhibition of the mature Npr by its own propeptide in the crude lysate was observed even in the absence of the covalent linkage between them. Pure precursor, propeptide and the mature Npr were isolated and kinetic parameters of the mature enzyme inhibition by the propeptide were determined. The inhibition is of the tight-binding competitive type with K _i 0.17 nM. Inhibition of metalloproteases from Brevibacillus megaterium and thermolysine by the heterologous propeptide of the Npr from B. brevis was much weaker or none.

【 授权许可】

Unknown