期刊论文详细信息
FEBS Letters | |
‘Red pigment’ from ADE‐2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases | |
Shlyapnikov, Mikhail2  Meškauskas, Arturas1  Ksenzenko, Vladimir2  Čitavičius, Donaldas1  Kryukov, Valentin2  | |
[1] Institute of Botany, Lithuanian SSR Academy of Sciences, Vilnius, 232027 LithuanianUSSR;Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of sciences, Pustchino on the Oka, 142292 Moscow region, USSR | |
关键词: ADE-2 locus; Red pigment; Restriction endonuclease; (S. cerevisiae); | |
DOI : 10.1016/0014-5793(85)80344-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Protection of DNA from cleavage by restriction endonucleases EcoRI, HindIII, BamHI, and Bg/II with red pigment, produced by ADE-2 mutants of Saccharomyces cerevisiae is demonstrated. Purification of yeast DNA from pigment can be achieved by chromatography on hydroxyapatite columns.
【 授权许可】
Unknown
【 预 览 】
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