‘Red pigment’ from ADE‐2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases

FEBS Letters

Shlyapnikov, Mikhail²  Meškauskas, Arturas¹  Ksenzenko, Vladimir²  Čitavičius, Donaldas¹  Kryukov, Valentin² 

[1] Institute of Botany, Lithuanian SSR Academy of Sciences, Vilnius, 232027 LithuanianUSSR;Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of sciences, Pustchino on the Oka, 142292 Moscow region, USSR

关键词: ADE-2 locus;    Red pigment;    Restriction endonuclease;    (S. cerevisiae);   

DOI  :  10.1016/0014-5793(85)80344-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protection of DNA from cleavage by restriction endonucleases EcoRI, HindIII, BamHI, and Bg/II with red pigment, produced by ADE-2 mutants of Saccharomyces cerevisiae is demonstrated. Purification of yeast DNA from pigment can be achieved by chromatography on hydroxyapatite columns.

【 授权许可】

Unknown