Ca2+‐dependent inhibition by trifluoperazine of the Na+‐Ca2+ carrier in mitoplasts derived from heart mitochondria

FEBS Letters

Crompton, M.¹  Hayat, L.H.¹ 

[1] Department of Biochemistry, University College London, Gower Street, London WC1E6BT, England

关键词: Heart;    Mitochondria;    Calmodulin;    Ca2+;    Na+;    Phenothiazine;   

DOI  :  10.1016/0014-5793(85)80316-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of trifluoperazine and extramitochondrial Ca²⁺ with the heart mitochondrial Na⁺-Ca²⁺ carrier has been investigated. External Ca²⁺ inhibits the carrier equally in mitochondria and mitoplasts in which the outer membrane is lysed. Sensitivity to Ca²⁺ is not removed by washing mitoplasts under varied conditions. Trifluoperazine is a potent inhibitor of the carrier in mitoplasts but not in mitochondria. Trifluoperazine inhibition in mitoplasts depends markedly on the presence of extramitochondrial Ca²⁺ (2 μM)

【 授权许可】

Unknown