Photo CIDNP study on the complex formation of λ‐ cro protein with OR3

FEBS Letters

Ikehara, M.¹  Kitano, K.¹  Lee, S.J.²  Shirakawa, M.²  Shin, M.¹  Kyogoku, Y.²  Akutsu, H.²  Ohtsuka, E.¹ 

[1] Faculty of Pharmaceutical Sciences, Osaka University, Suita 565, Japan;Institute for Protein Research, Osaka University, Suita 565, Japan

关键词: Photo CIDNP;    Cro protein;    Operator;    DNA-prolein interaction;    Nitration;    NMR;   

DOI  :  10.1016/0014-5793(85)80277-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A photo CIDNP spectrum of λ cro repressor protein showed that one of the three tyrosines and His 35 are quite accessible to the photosensitive dye. For the remaining two tyrosine residues one is slightly accessible, but the other is inaccessible. In comparison with the result of differential nitration at tyrosine side chains followed by the peptide analysis, it can be concluded that Tyr 26 is mostly exposed and Tyr 51 is slightly exposed on the surface of the cro dimer. On the addition of O_R3 17mer, His 35 and Tyr 26 are no longer accessible to the dye, which indicates that they are involved in interaction. However, a similar phenomenon was observed by adding CAP binding site 22mer. The interaction mechanism will be discussed.

【 授权许可】

Unknown