The effect of rhodopsin phosphorylation on the light‐dependent activation of phosphodiesterase from bovine rod outer segments

FEBS Letters

Dizhoor, A.M.¹  Arshavsky, V.Yu.¹  Shestakova, I.K.¹  Philippov, P.P.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Rhodopsin phosphorylation;    Light-dependent phosphodiesterase;    ROS;    rod outer segments;    PDE;    cyclic nucleotide phosphodiesterase;    TD;    transducin;   

DOI  :  10.1016/0014-5793(85)80272-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

ATP quenches light-dependent phosphodiesterase (PDE) activation in rod outer segments presumably due to rhodopsin phosphorylation. Here we compared the efficiency of phosphorylated and non-phosphorylated rhodopsins as PDE activators in a reconstituted cell-free system. It is shown that the ability of phosphorylated membranes to activate this enzyme is supressed compared with non-phosphorylated ones.

【 授权许可】

Unknown