Localization of light‐induced conformational changes in bovine rhodopsin

FEBS Letters

Virmaux, Noëlle¹  Nullans, Gérard¹  Pellicone, Chantai¹ 

[1] Centre de Neurochimie du CNRS - 5, Rue Blaise Pascal, 67084 Strasbourg Cedex, France

关键词: Conformational change;    Rhodopsin;    Photoreceptor;    CNBr cleavage;    Extradiscal region;    Dynamic structure;    G-protein;    GTP-binding protein or transducin;    ROS;    rod outer segments;    PAS;    periodic acid Schiff staining;   

DOI  :  10.1016/0014-5793(85)81138-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Conformational changes in the extradiscal regions of rhodopsin induced by illumination were investigated by modifying the visual pigment by mild treatment with cyanogen bromide prior to and after light exposure. Light induced an increased yield of cleavage of the Met bond 253-254 and a new cleavage at the Met bond 155–156 of the rhodopsin polypeptide chain. These residues, located at the beginnings of the membrane-buried helices 6 and 4, respectively, were concluded to become extradiscally exposed upon illumination.

【 授权许可】

Unknown