Localization and quantitation of opsin and transducin mRNAs in bovine retina by in situ hybridization histochemistry

FEBS Letters

Brann, Mark R.¹  Young, W.Scott¹ 

[1] Laboratory of Cell Biology, National Institute of Mental Health, Building 36, Room 3A-17, Bethesda, MD 20892, USA

关键词: Rhodopsin Transducin Hybridization (Retina) Photoreceptor Guanine nucleotide-binding protein;   

DOI  :  10.1016/0014-5793(86)81151-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oligodeoxynucleotide probes complementary to a portion of bovine opsin mRNA and transducin mRNA were used for in situ hybridization histochemistry. Within the retina, only photoreceptors expressed mRNAs detectable with these probes, and the majority of both mRNAs were in photoreceptor inner segments. More opsin mRNA was detected than transducin mRNA. In the inner segments 0.54 ± 0.05 copies/μm³ of opsin mRNA and 0.34 ± 0.05 copies/μm³ of transducin mRNA were detected. In the outer nuclear layer, 0.39 ± 0.06 copies/ μm³ of opsin mRNA and 0.27 ± 0.04 copies/μm³ of transducin mRNA were detected.

【 授权许可】

Unknown