Kinetic analysis of short‐term effects of α‐agonists on gluconeogenesis in isolated rat hepatocytes

FEBS Letters

Groen, A.K.¹  Verhoeven, A.J.¹  Leverve, X.M.¹  Tager, J.M.¹ 

[1] Section for Medical Enzymology and Metabolism, Laboratory of Biochemistry, University of Amsterdam, P.O. Box 20151, 1000 HD Amsterdam, The Netherlands

关键词: Gluconeogenesis;    α-Agonist;    Phenylephrine;    Malate-aspartate shuttle;    Hepatocyte;    Catecholamine;   

DOI  :  10.1016/0014-5793(85)81110-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isolated hepatocytes from fasted rats were perifused with glycerol as gluconeogenic substrate. Stimulation of gluconeogenesis with phenylephrine (10⁻⁵M) as α-adrenergic agonist consisted of two distinct phases. The first phase was a transient stimulation of gluconeogenesis and was accompanied by transient changes in cytosolic and mitochondrial redox state; this phase was abolished by the transaminase inhibitor aminooxyacetate. The second phase was a stable stimulation of less magnitude, without change in redox state and insensitive to addition of aminooxyacetate. It is concluded that the first phase is due to a transient enhancement of flux through the malate/aspartate shuttle and that the stable phase is probably due to a stimulation of mitochondrial glycerol-3-phosphate dehydrogenase and glycerol kinase.

【 授权许可】

Unknown