A novel immunoassay with direct relevance to protection against organophosphate poisoning

FEBS Letters

Ben-Zion, Sarit¹  Horowitz, Ora¹  Balan, Ayala¹  Torten, Michael¹  Heldman, Eliahu¹ 

[1] Israel Institute for Biological Research, POB 19, Ness-Ziona 70450, Israel

关键词: Organophosphate;    Immunization;    Acetylcholinesterase;    Paraoxon;    Antiparaoxon antibody;    Immunoassay;   

DOI  :  10.1016/0014-5793(85)81079-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding ofparaoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase against organophosphate poisoning. The toxicity of paraoxon was reduced in mice which were pre-injected with the immune sera.

【 授权许可】

Unknown