Thrombin induces the rapid formation of inositol bisphosphate and inositol trisphosphate in human platelets

FEBS Letters

Binder, Helge¹  Siess, Wolfgang¹ 

[1] Medizinische Klinik Innenstadt der Universität, Ziemssenstr. 1, 8000 München 2, FRG

关键词: Platelet activation;    Thrombin;    Phospholipase C;    Inositol phosphate;    Inositol phospholipid;    Ptdlns;    phosphatidylinositol;    PtdIns4P;    phosphatidylinositol 4-phosphate;    PtdIns4;    5P2;    phosphatidylinositol 4;    5-bisphosphate;    HPLC;    high-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(85)80241-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human platelets prelabeled with [³H]inositol were exposed to thrombin. The aqueous soluble inositol phosphates were separated by anion exchange column chromatography, paper chromatography or high-performance liquid chromatography, and identified by cochromatography with authentic standard substances. Thrombin immediately induces the rapid formation of inositol 1,4-bisphosphate and inositol 1,4,5-trisphosphate. Accumulation of inositol-1-monophosphate and inositol-2-monophosphate occurs later after a time lag of 10 sec. The results indicate that the phospholipase C induced polyphosphoinositide hydrolysis rather than the phosphatidylinositol hydrolysis is the triggering event for platelet activation, and support the concept of inositol 1,4,5-trisphosphate as putative second messenger.

【 授权许可】

Unknown